Intraoperative:
- At least 3 and optimally 5-6 samples of periprosthetic tissue must be obtained for:
- Histopathology of periprosthetic tissue.
- Periprosthetic tissue/pus culture.
- Sonication of the prosthetic implant will detect biofilm organisms &improve microbiological yield especially in patients with prior receipt of antimicrobials.
- Molecular methods and synovial fluid biomarkers are newer diagnostic modalities for culture negative PJI.