1. Samples: From pus or wound swab
2. Transport: Immediate transport to the lab
3. Culture:
• Aerobic: Blood agar, Mac Conkey agar, 37 0C in air
• Anaerobic: Brain Heart Infusion agar with hemin and vitamin K (anaerobic incubation): 37 0C, under anaerobic conditions
• Fungal culture (if needed): SDA with appropriate antibiotics/ BHI-BA with appropriate antibiotics and incubation temperatures (25 0C and/or 37 0C)

In culture confirmed cases of SSI/ soft tissue infections, antimicrobials should be based on lab AST reports